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For mHLA-DR measurement, staining and cell acquisition by flow cytometry (FC500, Beckman Coulter, Hialeah, FL, USA) were performed as described in the European standardized protocol and according to the manufacturer's recommendations (Quantibrite HLA-DR/Monocyte antibody cocktail, BD Bioscience, San Diego, CA, USA).
Cell acquisition was performed with CyAn-ADP (Beckman Coulter) using Summit 4.3.1 software (Dako).
CellQuest Pro software (Becton-Dickinson) was used for cell acquisition and analysis.
Cell acquisition was performed with FACSort Becton Dickinson in a region (R1) defined gating out only debris by forward and side scatter characteristics.
Immediately, prior to cell acquisition, 150 µL of TryPLE express (Invitrogen) was added to the cell suspension to avoid clotting in the CyAn.
Mean apoptotic cell acquisition upon addition of 4 µg\ml rHBD, (138 percent increase) was close to the effect of TSP-1 (162 percent increase).
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These molecular events lead to a less rigid cytoskeleton, reduced cell-cell contact, acquisition of cell-elongated shape, cell invasiveness, and metastasis.
A critical step in the development of a malignant cancer is tumor cells' acquisition of the capacity to migrate and invade tissues [1].
To uncoat the engulfed virion and to establish subsequent infection cycles as well as to spread infection to neighboring cells, acquisition of virus-cell/cell cell membrane fusion and enhancement of the cell–cell fusion activirus-cell/cell cellential step for successful selection/adaptation of virus to the new host cells.
Two separate cameras were used depending on whether it was live-cell acquisition (Hamamatsu ImagEM EM-CCD Camera C9100-13) or fixed samples (CoolSnap HQ camera, Photometrics).
Cells acquisition was performed on a FACSCalibur, LSR II or Canto II (BD) and analysed with the FlowJo software (TreeStar).
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