The gonocyte pluripotency marker OCT4 was expressed in most germ cells of first trimester testes prior to grafting, while after grafting some of the germ cells had ceased to express this protein, coincident with emerging expression of proteins associated with differentiation into pre-spermatogonia, such as MAGE-A4 and VASA (Fig. 4A).
We interpret the latter fate (NSC 'loss', her4:dRFP-positive RGs disappearing from the field of view within 72 h and not re-expressing her4:dRFP for at least 7 days) as RG cells that ceased to express her4 without dividing (no mcm5:eGFP expression was detected), suggesting that they may have directly transformed into another cell type.
The hope seems to be that if sufficient ammunition is arrayed against the markets, then they'll cease to express their lack of confidence in a successful resolution of the euro-zone crisis.
Throughout Murakami's oeuvre, on the other hand, his characters never cease to express their bafflement about the nature of time, or change, or consciousness, or moral choice, or the simple fact of finding themselves alive, in this world or another.
Culturing corneal keratocytes is difficult because keratocytes growing in a monolayer rapidly lose their stellate morphology and cease to express keratocyte markers such as keratocan, lumican and aldehyde dehydrogenase 1 family, member A1 (A1DH1A1).
The world has ceased to express its ideals in verse".
Type-3 progenitor cells, that cease to express nestin but still express DCX [52], significantly increased in number in PC3/Tis21-null mice (BrdU+/nestin−/DCX+; about 17% increase; Figure 1D).
GABAergic interneurons were observed to migrate from subpallium to pallium in these explants but had ceased to express ASCL1 on leaving the ventral proliferative zones.
Second, we did not observe widespread loss of H2A-mTurquoise, which would be expected if Schwann cells ceased to express Gal4 after denervation (Fig. 4B-D).
Most of the tumors were lobular in morphology and they progressively ceased to express receptors after a few in vivo passages.
