Remarkably, 19% of expandable clones (9 of 43) could continue proliferating further over 2 months with morphological change to doom-like colonies resulting from tight cell-to-cell association, whereas residual clones with fibroblast-like feature gradually ceased cell proliferation.
By the beginning of gastrulation (E6.5), stem cell proliferation ceases and differentiation into the three germ layers is initiated.
The activity of TOR is switched off and cell proliferation ceases under conditions of low concentrations of amino acids or when energy is limiting.
These CHX-treated cells seemed to cease proliferation completely; a cell proliferation assay showed that the normally fast-growing C6 cell no longer proliferated when treated with LCC.
Upon overexpression of the LETF genes, cell proliferation ceased and the cells exhibited an epithelial morphology, indicating hepatic maturation of hepatoma cells.
In the presence of the GSK-3 inhibitors, cell proliferation ceased and apoptosis was induced in all tumour cell lines that we investigated.
Consistent with this hypothesis, our temperature shift experiments indicated that the let-418 activity must be present in the embryos before reaching the bean stage (i.e. when cell proliferation largely ceases and morphogenesis begins) in order to prevent L1 larval arrest.
Taken together, our results indicated that, in order to bypass the L1 larval arrest, the let-418 activity is required prior to the lima bean stage, which corresponds to the time point when embryonic cell proliferation largely ceases and morphogenesis starts [37].
This is in marked contrast to the rodent in which more synchronous germ cell differentiation occurs in late fetal and early postnatal life, during a relatively narrow time window and germ cell proliferation completely ceases during late gestation and early postnatal life.
That is, when the G′ value of the hydrogel was higher than 1.0 kPa, the cell proliferation was ceased and the proliferation cycle of cells was converged to G1 phase, whereas when the G′ value was below 1.0 kPa, cell proliferation proceeded.
