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Because CDS length does not also negatively correlate with the evolvability index, we conclude that CDS length cannot be responsible for the observed associations between the evolvability index and structural modularity and robustness.
CDS length was also key factor in dictating codon usage variation.
A general tendency of more biased codon usage of genes with longer CDS length to higher expression level was found.
No statistically significant correlation was found between exon length and CDS length (Fig. S2).
No statistically significant correlation was observed between mean exon length and mean CDS length (P = 0.132; Fig. S2).
The exon/gene ratio also increases (Fig. 1B), but the mean and median exon length becomes shorter (Fig. 1C), whereas CDS length remains relatively constant.
The median CDS length is 927 bp, while the average length of 5'-UTR and 3'-UTR is about 234 bp and 451 bp respectively.
Instead, CDS length had the strongest effect on dN (or dN/ dS) among all the gene properties examined (CDS Length vs. dN: ρ = 0.552, P < 10−; CDS Length and dN/ dS: ρ = 0.403, P < 10−) (3,480 orthologs, table 1).
In contrast to CDS length, tomato nrFLcDNAs had longer UTRs compared to those of other plants.
Young orphan genes are more likely to be lost: accounting for CDS length.
In contrast, CDS length had a positive coefficient for ribosome occupancy.
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