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These two transcripts were identified by cDNA sequencing of RT-PCR products.
We also discuss how to test RNA editing in vivo by cDNA sequencing.
We present the "hyb" bioinformatics pipeline, which we developed to analyse high-throughput cDNA sequencing data from CLASH experiments.
In searching the data bases we identified the product of a cDNA sequencing project that bears significant similarity to MRP subfamily transporters.
Brain-derived DNA complementary to RNA (cDNA) sequencing predicts a 154-amino acid product with no similarity to any known protein.
Based on N-terminal and conserved internal protein sequences, primers were designed and used for PCR gene amplification and cDNA sequencing.
However, since cDNA sequencing is still ongoing sequence information remains incomplete.
Of these, 25% were not detected by cDNA sequencing of blood or sporozoite stages.
PALD (KIAA1274) was identified in large-scale cDNA sequencing efforts [13].
The DNA mutations were confirmed by alternative methods including restriction enzyme digestion (for A15T) and cDNA sequencing (for R217H).
This was confirmed after cDNA sequencing.
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