Sentence examples for cdna in from inspiring English sources

This was followed by amplification of the entire cDNA in five overlapping amplicons.

To investigate the involvement of CYPs with the metabolism of dioxins, we first searched for CYP cDNA in P. brevispora.

The presence of TcTrx cDNA in the selected transformants was verified by PCR using gene-specific flanking primers.

Expression of a fc177 cDNA in dec-1 null mutants was sufficient to restore spaces within the endochorion layer.

For overexpression in rice, the XB21 cDNA in pENTRTM/D-TOPO® was recombined into an Ubi-1300 vector using Gateway® LR Clonase (Invitrogen).

The cDNA was checked by PCR using Phusion polymerase (NEB, Hitchin, UK) with 0.5 μl of cDNA in a 20 μl PCR.

Subsequently, 20 μL purified RNA was reversely transcribed into cDNA in a 40-μL reaction system according to the manufacturer's instructions.

Subsequently, 1 µg of total RNA was reverse-transcribed into cDNA in a 20 µl volume using the SuperScript™II Reverse Transcriptase (Invitrogen) by following the manufacturer protocol.

Two μg of total RNA was reverse-transcribed into cDNA in a 20-μL reaction mixture using a PrimeScript Reverse Transcriptase kit (Takara).

Negative controls were performed without cDNA in the reaction mixture.

We initially detected the ADAR2R cDNA in mouse brain.