Exact(1)
Briefly, mouse fibroblast L929 cell line (CCL 1) (American Type Culture Collection, Manassas, VA, USA) was cultured in Dulbecco's modified Eagle's medium containing 10% fetal calf serum.
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The cell culture studies (MTT assay, Confocal Laser Scanning Microscope (CLSM) and SEM analyses) carried out with L929 ATCC CCL-1 mouse fibroblast cell line proved that the biocompatibility performance of the scaffolds.
Murine L929 (ATCC N° CCL-1) is a fibroblastic-like cell line.
injection of CCL-1 on nociceptive behaviour.
CCR-8 is a specific receptor for CCL-1.
And we showed that CCL-1 (25 ng, i.t).
In addition, we observed that CCL-1 (25 ng, i.t).
Recombinant mouse CCL-1 and neutralizing antibody CCL-1 were purchased from R&D Systems (Minneapolis, MN, USA).
In the CCL-1-sensitive cells, the frequency and amplitude of sEPSCs in the presence of CCL-1 were 174±11% and 110±6.3% of controls, respectively.
CCL-1 induced phosphorylation of NR2B subunit at Tyr1472 (p-NR2B) in the spinal cord at 1 and 3 h after injection of CCL-1 (25 ng, i.t).
However, increased expression of CCL-1 was observed by in situ hybridization by another group [ 28].
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