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A 2,300 bp promoter region of PPARγ2 gene was amplified by polymerase chain reaction (PCR) using a set of primers 5'- TCC CCG GGG GTA TGT GG A GCC CA A CCC A -3' and 5'- ACG TCG ACA GAT TTG CTG TAA TTC ACA CTG G -3' containing SmaI and SalI sites and the resultant product was subcloned into pluc4 luciferase reporter construct and confirmed by subsequent analysis [78], [79].
The CCC macro is presented for computation of the concordance correlation coefficient (CCC), a common measure of reproducibility.
Earlier Thursday, it cut the $3.2 billion sewer debt three levels to CCC, a noninvestment grade.
In February, Standard & Poor's lowered its credit rating to CCC, a negative outlook.
The rating of the ACA Financial Guaranty Corporation was cut to CCC, a subinvestment grade, from an A rating.
According to figures from the Committee on Climate Change (CCC), a government-funded but independent advisory body, the waste, road transport and power sectors are performing particularly well.
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Mutagenesis primers used were as follows: 5′-GGT CCC CGG CCC CAG GCT AAC GGG TCA CTT CAC CCC A-3′ and 5′-TGG GGT GAA GTG ACC CGT TAG CCT GGG GCC GGG GAC C-3′.
The un-methylated specific primers (USP, U-reaction) that specifically hybridized with the un-methylated Id4 promoter sequence were forward 5′ (−194 to −166 bp) GGT AGT TGG ATT TTT TGT TTT TTA GTA TT-3′ and reverse 5′ (−60 to −33 bp) AAC TAT ATT TAT AAA ACC ATA CAC CCC A-3′ (reverse).
Ben Caldecott is Head of UK & EU policy at Climate Change Capital (CCC), an environmental investment manager and adviser.
The present study examined driver's ability to distinguish between different compliance rates to CCC, an advisory driver support system aimed at improving traffic flow.
Thirty percent of infected individuals develop chronic Chagas cardiomyopathy (CCC), an inflammatory dilated cardiomyopathy that is, by far, the most important clinical consequence of T. cruzi infection.
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