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To see whether Ubx prevents the death of CCAP-ENs by counteracting a possible action of the anterior-most HOX genes in promoting apoptosis of the CCAP-ENs, we stained for CCAP in mutants of labial (lab / lab ), proboscipedia (pb ), Deformed (Dfd ), Sex combs reduced (Scr ) and Antennapedia (Antp / Antp ), but did not observe any phenotype (supplementary material Fig. S3A-E).
The Center for College Affordability and Productivity (CCAP), in conjunction with Forbes, compiled its college rankings using five general categories, with several components within each general category.
According to the Illinois Comptroller, in fiscal year 2014, the state provided $312.5 million to CCAP; in fiscal year 2015, the state appropriated $228.4 million, despite telling child care providers to continue operating at the same clip as the previous year.
We also generated variants of peptide ccAA in which the CC propensity was enhanced by the insertion of CC-stabilizing hydrophobic residues (peptide ccAL) or diminished by CC-disrupting prolines (peptide ccAP) in a/d heptad positions.
Furthermore, CC-stabilizing (peptide ccAL) and -destabilizing mutations (peptide ccAP) in the polyA stretch, respectively, enhance or abolish aggregation, similarly to what was observed with similar mutations in full-length and ccd RUNX2 (Fig. 6F).
The organelle DNA sequence data presented in this study come from D. salina strain CCAP 19/18, which is maintained at the Culture Collection of Algae and Protozoa (CCAP) in Argyll, Scotland [ 66].
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There are two types of CCAP neurons in the ventral nerve cord (VNC) of the fly: interneurons (CCAP-INs) and efferent neurons (CCAP-ENs).
In the null allele Ubx 6.28 the CCAP-ENs of T3 A1 were lost, although the CCAP-INs were not affected; on the other hand there was no change in the pattern of CCAP neurons in the null allele abd-A M1 (Fig. 2E-F,I; supplementary material Table S1).
We observed one extra CCAP-EN per hemisegment in segments SE1-T2, while the fate of the CCAP-INs was unaffected (Fig. 3A-A′,G).
Second, Abd-B is not required for specification of these neurons, but temporally represses expression of the neuropeptides in the CCAP-ENs of segments A5 7, without affecting their expression in the CCAP-INs.
As CCAP-ENs cannot be identified in segments A5 7 in stage 17, we directed our attention to the expression of Abd-B in CCAP-INs at different times of development and observed that Abd-B was never expressed (supplementary material Fig. S4A-C).
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