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In addition, the stringent definition of clonal complexes (CCs), with which strains sharing at least 6 identical alleles are grouped into the same CC, was applied.
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A central composite (CC) design was applied as a tool to obtain a stable and low viscosity emulsion.
In this paper, the combined consolidation (CC) method was applied to a site with loosely deposited soft soils in the Yangtze River Delta of China.
Lucrin was diluted in the IVF unit (1 cc Lucrin corresponding to 10 cc SF) and was applied in both the morning and evening (2 × 40 µg) subcutaneously (SC).
Finally, same cross correlation (CC) analysis as described before was applied to the MC simulated traces for calibration purpose (τ, dwell time of CC analysis; D, diffusion coefficient).
A model, based on the Cohen-Turnbull-Fujita (CTF) model, considering the polymers to consist of four components, CC, ICC, IL and L, was applied to the diffusivity data.
To calculate the CC features, the Hanning window procedure was applied to each analysis window of the EMG signals.
E3 (9.3 g) was applied to SiO2 CC with an eluent of CHCl3 MeOH (2:1) and further purified through HPLC (40% MeOH) to provide compounds 14 (6 mg) and 15 (26 mg).
D2 was applied to SiO2 CC with an elution of CHCl3 MeOH (1:2–0:1), and then purified by silica gel and HPLC to give compounds 4 (10 mg), 5 (4 mg), 6 (8 mg) and 9 (7 mg).
The subfraction F4.2 displayed significant spots and was applied to SiO2 CC, eluted with n-hexane and acetone gradients (100 : 1 to 1 : 1), to afford β-amyrin acetate (2) (10 mg) and taraxasterol acetate (3) (6 mg).
Tubes were snap-sealed and vacuum was applied using a 20 cc syringe and incubated at 37°C for 3 hrs.
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