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At each of the ten sites, four pitfall traps (300 cc), containing a solution of 90% ethanol and detergent, were buried in the ground for walking or crawling herbivores to fall down.
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While there was no association of class II pilE with serogroup, country or year, the presence of a class II pilE gene was consistently associated with a limited group of clonal complexes i.e. cc1, cc5, cc8, cc11 and cc174; no WGS data from isolates belonging to these ccs contained a class I pilE gene.
Briefly, PBMCs were cultured (107 cells/ml) in 6-well plates for 18 to 36 h in complete medium in the presence of inflammatory cytokines (a cytokine cocktail (CC) containing IL-1β (10 ng/ml), IL-6 (10 ng/ml), TNF-α (10 ng/ml) plus PGE2 (1 μg/ml)), poly I C (50 μg/ml) or CD40L (2 μg/ml) and subsequently stained for flow cytometric analysis.
The CC containing millions of evenly distributed, uniformly sized macroscopic air-voids of approximately 0.1 1 mm in size is considered to have good freeze-thaw (F-T) resistance.
The TM has increased from 1,472 to 2,190 MPa for pure PVA film to 9 % CC containing composite.
The resulting powder was subjected to column chromatography (CC) containing 47 g of silica gel.
Each DL/UL transmission subframe for a user on a CC contains several subcarriers over several MA symbols forming a resource grid (RG).
In cases where a CC contains only two STs, the lower numbered ST was used to define the CC.
To determine whether the presence of cysteine residues is sufficient to allow for functional activation in the absence of tyrosine phosphorylation, we used a STAT1-CC mutant containing an Y701F substitution.
The Murchison CC contains small amounts of straight-chain fatty acids, though some of these may be contamination (Yuen and Kvenvolden 1973).
The Z′ = 3 structure of 1-I (stable form; space group Cc) contains local symmetry elements.
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