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A total of 353 CBs >1 kb were recognized.
To determine the expression pattern and subcellular localization of cbs-1, we constructed the translational vector cbs-1 GFP, cbs-1 GFPtains the promoter and the entire CBS-1 sequence tagged at the C-terminus whichGFP.
To confirm the efficacy of cbs-1 RNAi, we measured the amounts of CBS-1 andigen and CBS activity in worm extracts of CBS-1-inactivated and WT worms.
To explore the functional significance of cbs-1 in C. elegans, we silenced the cbs-1 gene by RNA-mediated interference and determined the phenotypic consequences of such silencing.
In our CBS-1 GFP CBS-1 GFPion study, CBS-1-knockdown worms exhibited abnormalocalization of studyal tissues that express the CBS-1-knockdown
In accordance with the recommended nomenclature, this gene was named cbs-1.
Expression of cbs-1 has also been observed in pharyngeal muscles and hypodermis.
We determined the tissue and subcellular distribution of CBS-1 and showed that cbs-1 knockdown by RNA interference leads to delayed development and to an approximately 10-fold elevation of homocysteine concentrations in nematode extracts.
Our data using a translational reporter showed a similar expression pattern, as did previous transcriptional screens, and a novel expression of cbs-1 in pharyngeal muscles.
To determine the expression pattern of cbs-1, we generated a translational fusion vector using the PCR fusion technique described previously [ 22].
We hypothesize that the intestinal expression of cbs-1 in worms may serve similar purposes, namely removal of homocysteine or cysteine biosynthesis.
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