Members of CBM families 3, 4, 6, 13, 32, and 48 were also identified.
Some CAZy families were only found in one mangrove: GH 6, 45, 70, 71, 72, and CBM 4 were only found in the RJ sample, while GH 11, 46, 79, 81, CE 5, and CBMs 15, 25, and 33 were only found in the BA sample.
F. succinogenes also appears to employ 'atypical' cellulases that may obviate the need for extensive CBMs [29].
The AA1 family was surprisingly found to be associated to various CBMs (CBM1, CBM18 and CBM20), which is singular for multicopper oxidases.
However, there was not a significant difference between groups in the number of CBMs (0 or >0) during the run-in period (P=0.2869).
Clade II is primarily composed of Actinobacteria and separates into subclades A and B. Subclades A and B contain only cellulose-binding CBMs (CBM2 and CBM3) associated with the catalytic AA10 domain, whereas subclades C and D contain only chitin-binding CBMs (CBM5 and 12).
Moreover, we repeated the experiment with five-fold less Cel9A-CBM (1 μM) and the final profile was nearly the same as at 5 μM (data not shown).
In addition, a non-enzymatic class exists, the carbohydrate-binding modules (CBMs) [ 11, 12].
OSA-CBM [13] is an implementation of ISO-13374.
Each scenario was simulated 100 times with the Carbon Budget Model of the Canadian Forest Sector (CBM-CFS3).
At the regenerating clearcut, unweighted ΔC2 was within 1 standard deviation of the CBM-CFS3 estimates of ΣNEP, and closest to unweighted CBM-CFS3 ΣNEP.
