We were, nevertheless, able to investigate the level of CB-specific immune selection against the CB allele at the msp-1 and ama-1 loci using RTQ-PCR.
Briefly, CD34+ cells were purified from CB by positive selection using the midi-MACS immunomagnetic separation system according to the manufacturer's instructions (Miltenyi Biotec, Bergisch Gladabach, Germany).
CB-pyr10 is a pyrimethamine resistant cloned line derived through pyrimethamine selection from CB (a pyrimethamine sensitive line), originally cloned from isolate CB [40].
Genomic selection for CB performance can significantly increase rates of genetic gain in crossbreeding programs.
Therefore, it seems reasonable to recommend the use of performance and genotypic data on CB individuals for genomic selection in crossbreeding programs.
Moreover, selection for CB performance is either on half-sibs, which does not exploit variation within the half-sib family, or on progeny information, which would lengthen the generation interval if it was used.
The main focus of this paper was to look at the effect of different proportions of PB and CB animals in the reference population, when applying genomic selection in CB programs, and these would not be much affected by the Bulmer effect.
The efficiency of investing in CB information to enable genomic selection of PB animals for CB performance was explored by varying the proportion of PB and CB animals in a reference population, or by adding CB individuals to an existing PB reference population.
The pot-1:: mCherry transgene was constructed using the Invitrogen Gateway Cloning kit using the positive selection marker Cb-unc-119, a germline-specific promoter daz-1, full-length genomic pot-1 sequence lacking a stop codon, mCherry sequence, and the tbb-2 3′ UTR.
The outcome is a first step toward determining the optimal genomic selection scenario to select PB for CB performance.
In a reciprocal experiment the uncloned progeny of the cross between P. c. chabaudi strains CB and AJ were subjected to CB-specific immune selection.
