The fact that those differences might have caused the template normalization to behave differently in the two groups cannot be ruled out.
Because the fluorescence of 2-AP within DNA is quenched by stacking with its immediate neighbors, this initial step would therefore involve a structural transition that simultaneously causes the templating base to become more stacked and its 5′ neighbor to become less stacked (in the case of templates C, G, and T, but not A).
In this process, Au NRs are deposited on the surface of a PNIPAM hydrogel, and the temperature is increased, causing the hydrogel template to shrink, thereby driving the Au NRs closer to each other.
Limiting the chase lowers the rate of nucleotide addition through dTTP regions on the template, causing the accumulation of transcripts that are ∼250 nt in length.
Short pieces of RNA, called microRNAs, control protein production by causing the proteins' RNA templates (known as messenger RNA or mRNA) to be disabled by the cell, according to Whitehead Institute scientists.
It is well known that long stretches of identical nucleotides in the template strand may cause a "stuttering" behaviour of the Taq DNA polymerase reducing the speed of the elongation process or causing the enzme to fall off the template strand.
We also ruled out the issue of secondary structure causing the 3' end of our Test Template to become inaccessible.
The thickened silica wall of solvothermally treated MMSS (MS) prevented the shrinkage of the mesoporous silica template during carbonization, causing the formation of nanospaces between carbon and the silica.
As the template caused the formation of primary macropores the activation created a secondary system of micropores which resulted in a significant increase of the specific surface area up to about 1400 m2 g−1.
A lateral wet-chemical etching of the ZnO template causes the InGaN/GaN bilayer film to peel off with a rational cracking in parallel to [112¯0] while perpendicular to [11¯00] and [0001].
