One inconsistency was caused by a requirement for a cofactor that could not be modeled.
Another example are the carAB genes: even though these genes are not characterized in C. glutamicum they are well known to cause a double requirement for both arginine and uracil in E. coli since they encode for the subunits of a protein that provides the essential carbamoylphosphate for arginine and pyrimidine biosynthesis [ 39].
Considering the prominence of ideas about a good cause as a requirement for sample re-use, we spent considerable time exploring what participants would consider a good cause.
For most plants, the accumulation of biomass accompanying shoot elongation causes an increased requirement for support (Rowe and Speck, 2004).
Although analysis of mouse mutants has not supported a role for single-gene mutations in FOCM as major causes of NTDs, a requirement for cellular uptake of folate for neural tube closure has been demonstrated in Folr1 null embryos, in which NTDs occur when rescued from early lethality by folic acid supplementation (36).
Recent studies using transgenic approaches (for review [ 29]) found that disruption of the MKK4/SEK1 gene caused embryonic death in mice, demonstrating a requirement for MKK4/SEK1 in development.
Delayed graft function (DGF) caused by severe ATN, defined by a requirement for dialysis during the initial post-transplant week, complicates postoperative management, and if prolonged (>14 days), adversely effects allograft survival.
Re-feeding causes germ cells to exit quiescence rapidly, and a requirement for GLP-1/Notch signaling similarly resumes.
Moreover, An. stephensi saliva causes DC migration and directly triggers mast cell degranulation without a requirement for IgE [3].
Moreover, TRAPS-associated TNF-R1 mutants cause enhanced production of mitochondrial ROS, which is a requirement for NLRP3 inflammasome activation.
