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Our analysis shows that nesting of the DysF has not caused sequence divergence in either the inner or outer DysF regions.
Also, it is important to note that the inversion might have caused sequence deletions/duplications/insertions, thus complicating the pre-insertion site amplification analysis.
In samples 00800-SD1 1 and 04011685, the same base insertion in exon 4 caused sequence frameshift responsible for the substitution of cysteine in position 135 to leucine, together with the premature termination immediately at codon 136.
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Concurrent timing caused sequencing judgments to become slower, less accurate, and less sensitive relative to sequencing-only conditions.
These results suggest that the integration of shRNA-CX3 or -CX5 into the genome of these transgenic rice plants has caused sequence-specific interference with homologous OsCKX2 expression withouting affecting the expression of other CKX genes.
Ago2 immunoprecipitated from nuclear extracts of cells treated with siLuc caused sequence-specific cleavage of the Luc substrate.
Interestingly, in the second study, neocentromere formation caused sequences adjacent to the CENP-A domain to shift to a later replication timing.
Recombination can also cause sequence duplications and deletions, resulting in rapid and sometimes substantial shifts in genome size.
As time passes, nucleotide substitutions cause sequence divergence between the two LTRs.
The efficiency of cleavage may be affected by tissue type or by mutations that cause sequence alterations [10].
INDELs play a major role in causing sequence divergence between closely related DNA sequences in animal, plants, insects and bacteria [ 12].
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