The charge engineered variant's two mutated amino acids exhibit stabilizing interactions with adjacent native residues, and from a global perspective, the mutations cause no gross structural perturbations or loss of stability.
In contrast, the M185A substitution caused no gross perturbation of the whole dimeric structure, only subtle changes in the interaction sites.
The similarity between the reaction kinetics of PSI reduction together with the similar cellular growth rates of wild-type and PSIPsaJF strains shows that deleting the N-terminus of PsaF causes no gross perturbation to the interaction between PSI and CytC553, that is, the N-terminus of PsaF does not play any positive role in this interaction in Synechocystis.
Two crystal structures of P411 variants of P450BM3 show that S400 coordinates the iron and causes no significant structural perturbation in the substrate binding pocket.
As described above, mutations of the other three histidines (H34A, H135A, and H221A) caused almost no perturbations in the N H stretching vibrational modes in the 2800 2400 cm–1 domain.
This result suggests that the co-seismic slip associated with the 17 August event caused no considerable stress perturbations in the western part of the Gulf.
The trend of the σ1 axis around the Yalova segment is close to the pre-Izmit mainshock trend of the maximum compressive stress axis, implying that the 17 August event caused no considerable stress perturbations in the western part of the Gulf of Izmit.
Importantly, the METTL3/METTL14 heterodimer harboring D334C/C336S mutations has no loss of activity (Fig. 3A), and the conjugation of the paramagnetic probe causes no further chemical shift perturbations.
In their models, perturbed processes cause the perturbation of the expression of individual genes belonging to each of those processes.
Also, no information is available with regard to whether the drug may cause a perturbation of epigenetic stability in any organism.
