Similar results were obtained with a ShNur 77 (i.e. ShNur 77-26) that caused no alteration in Nur 77 expression (data not shown).
In contrast, administration of EPO to endotoxemic βcR KO mice caused no alteration in IL-1β expression (P>0.05) (Fig. 3E).
Individual mutations of the Fkh1-, Fkh2- or Fkh3-binding sites in the Ndg enhancer caused no alteration in reporter expression in somatic myoblasts (data not shown).
In both cases, the changed nucleotide is in the 3rd coded position, which may influence the fact that it causes no alteration in the amino acid sequence.
By contrast, SAGP caused no alteration in labelling the alpha-subunit of stimulatory guanine nucleotide-binding (Gs) protein in a subsequent CTX-catalysed ADP ribosylation of membrane fractions of Meth A and L929 cells.
If a PCR product did not show any discrete alteration on the gel, the mutant was classified as one containing a point mutation with either a base substitution or a frameshift causing no alteration in the size of the gene product.
At 48 h, basal levels of NO increased in uninfected macrophages, GMFC being 79.65±3.08, but Berberine chloride caused no alterations (Figure 2C).
The polymorphisms in the H2 haplotype are either located in intronic regions of the gene or were silent, i.e. causing no alterations of the P2Y12 receptor protein.
In contrast, EG3287 caused no alterations in the cytotoxic effects of 5-FU in breast carcinoma MDA-MB-453 cells, which are NRP1-negative.
Attachment of the accelerometers caused no alterations of normal behaviour although some horses proceeded to roll shortly after attachment, but quickly became accustomed to the device.
