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The presence of poly(A) tails in assembled contigs can cause misalignments to the reference genome.
As a result, any variants near the amplicon boundaries can cause misalignments of multiple reads that can ultimately lead to false-positive or false-negative variant calls.
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This "missing wedge bias" towards favoring larger density overlaps can cause misalignment of subtomograms; however, it can be compensated for by cross correlation map normalization or other methods.
Anything in an organization that causes a deviation from the vision or a given directive will cause misalignment in the tires of the operation.
Thus, protruding hairpin structures cause misalignment of template and compliment strands, which subsequently do not tether to form a stable duplex.
Because the elements of each family are numerous and have highly homologous sequences, they may cause misalignment and serve as homology breakpoints for non-allelic homologous recombination (NAHR).
We used a total of 13 vertebrate genomes with over 6X coverage and high quality since alignments containing low-coverage genomes cause misalignment.
Finally, our PET/MRI system acquires the PET and MRI simultaneously allowing for perfect alignment between the 2 sets of images, when compared with the sequential acquisition in PET/CT where minor head movements can cause misalignment.
It should be noted that the alignments of some of these genes contained substantial indel polymorphism (particularly ORF clusters 66, 84, and 95) that can cause misalignment and an inflated estimate of the number of nonsynonymous mutations.
This variation causes misalignments between reads arising from the same genomic region, preventing correct assembly by most algorithms.
The update time interval during tracking causes misalignment of the lens' optical axis versus the sunrays.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com