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A caudal cut is then made.
The cerebellum was removed from the brain with a further caudal cut along the dorsoventral axis.
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For further analysis of Grhl2 expression in Axd and Axd/Grhl2 GT embryos, RNA was purified from isolated caudal regions (cut at the level of somite 21), genomic DNA removed by DNase I digestion (DNA-free, Ambion) and first-strand cDNA synthesis performed (SuperScript VILO cDNA Synthesis Kit, Invitrogen).
The trachea was cut caudal to the larynx and cranial to the bifurcation.
Briefly, the brains were placed ventral side up, and 4-mm-thick coronal slices were cut caudal to the optic chiasm.
We cut caudal and anal fins from 1000 adult fish, and dissociated the pigment cells.
The caudal vein was cut immediately and the rats were sacrificed by exsanguination still being under deep anesthesia by isoflurane.
For imaging caudal-fin neuromasts, adult zebrafish were anesthetized with a 610 µM solution of MS-222 and caudal fins were cut with a sharp scalpel.
It consists of a series of eight caudal vertebrae in a cut slab of carbonate mudstone, which was judged to date from the late Hettangian to Sinemurian stages.
In a normal tail detached from the sixth caudal vertebra, and averagely cut into four segments, CD59 transcript levels presented no changes between each sample.
With the previous transverse cut, set caudal to the genu of the corpus callosum, both hemispheric parts stay connected, which facilitates cutting and processing.
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