The two most frequently used methods for pregnancy diagnosis of cattle are manual palpation of the reproductive tract (per rectum) and transrectal ultrasonography of the reproductive tract [ 2].
To elucidate the morphological differences between placentas from normal and cloned cattle pregnancies reaching term, the umbilical cord, placentomes and interplacentomal region of the fetal membranes were examined macroscopically as well as by light and scanning electron microscopy.
In contrast to these reports, we obtained live, actively proliferating and normal cells from an organ that had been collected from a dead animal and frozen in a −80°C freezer for 10 years, and we produced four viable cloned cattle from five pregnancies by a conventional single-step SCNT technique.
The objective is to achieve sensitivity and specificity in excess of 95% across the range of pregnancies, cattle and facilities found on commercial farms whilst operating at speeds of up to 60 cows per hour.
Two experiments were designed to evaluate models for generation of low circulating progesterone concentrations during early pregnancy in cattle.
Given the suggestion that the bacterium recrudesces during pregnancy in cattle, this study was designed to determine whether C. burnetii infection affects hormonal patterns, such as progesterone, cortisol, pregnancy associated glycoproteins (PAG), and prolactin during gestation in lactating cows.
The isolation and characterization of pregnancy-specific protein B (PSPB) [ 1] or bovine pregnancy-associated glycoprotein 1 (bPAG1)[ 2] in the cow by immunoelectrophoresis and the subsequent development of RIA techniques for this protein (for PSPB[ 3] and for bPAG1[ 4]) enabled hormonal diagnosis of pregnancy in cattle.
Pregnancy testing in cattle is commonly invasive requiring manual rectal palpation of the reproductive tract that presents risks to the operator and pregnancy.
Taken together, the combined results suggested that PGF2α could be successfully used to induce and synchronize ovulation in cattle undergoing TAI, with similar pregnancy rates when compared with other ovulatory stimuli (ECP and EB).
The desired target sensitivity and specificity for a commercial pregnancy test in cattle is 95% or greater for both.
