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feRPS5 mRNA expression was consistently detected at similar levels between tissues and individual cats (data not shown).
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We also confirmed that CRMP-2 was phosphorylated by PKN-cat (data not shown).
The expression of Bsep was observed in livers from rat, dog and cat (data not shown).
As further evidence for the emergence of D. gatoi in Finland, the authors (SS, LSK, KJ and RLS) - since writing this paper - have diagnosed additional cases (data not shown) of D. gatoi-associated pruritic skin disease in several pedigree cats (e.g. in Russian Blue cats).
We also found no evidence that co-transfected ORF75c transactivates the ORF50 promoter in CAT assays (data not shown).
Upon growth on Glc/AS, Glc/MA or Gly/AS, mGFP-Yap1 was predominantly localized to the cytosol in WT and cat cells (data not shown).
Dose-dependent inhibition of IL-13/TNFα/TGFβ1-stimulated eotaxin-1 production showed that the process was IL-13 dependent with an IC50 of 32.4 nM CAT-354 (data not shown).
The 5'splicing sites (GT) and 3'splicing sites (AG) were conserved among mammalian rhodopsins (except one site in dog and one site in cat, intron data not shown), suggesting the presence of selection on splicing control for introns.
In contrast, no such significant difference was observed for cats and pigs (data not shown).
CgA-derived fragments in the PMN secretions obtained after treatment with CHR and CAT were detected by western blotting, revealing traces of Vasostatin-1 and a CAT-including fragment (CgA340 395) (data not shown), consistent with the previously report of several CAT-including peptides with molecular weights in the range 70-15 kDa [17].
Similar mouse MMP-11 fragmentsgments were generated by Cat-MMP-14 cleavage (data not shown).
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