Staining was categorised into four grades according to immunohistochemical scores.
Clefts of the palate are generally categorised into four grades (Fig. 1): grade 1, submucous cleft/bifid uvula; grade 2, soft palate only; grade 3, soft palate and <1/3 hard palate; grade 4, complete CP [ 17].
Although high gene copy numbers are found in plants and deuterostome animals, the superfamily has been categorised into four major grades consisting of classical water-selective type aquaporins (Aqp0, −1, −2, −4, −5, -5-like, −6, −14, and −15), Aqp8-type aquaamoniaporins (Aqp8 and −16), aquaglyceroporins (Glp, Aqp3, −7, 9, −10 and −13) and unorthodox aquaporins (Aqp11 and −12) [ 9– 13].
Histologic grade was categorised into three groups: (1) Gleason Score 5 and 6; (2) Gleason Score 7 and (3) Gleason Score 8 10.
Employment grades were categorised into three groups: administrative, professional, or executive; clerical; and other unskilled manual jobs.
Data on the grade of surgeon and anaesthetist was categorised into two groups: consultant grade or other (including all types of trainees and staff grade doctors).
All factors were used as dichotomous covariates in the statistical analysis with the exception of grade and the NPI, which were categorised into three groups.
