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These shared genes could be divided in four main categories: regulation of cell cycle progression and proliferation (ex.
These genes cluster into 5 major categories: regulation of transcription, transport, signal transduction, protein turnover, and metabolism.
In particular, the GO categories "regulation of cellular process" and "cell differentiation" were significantly affected by MMG.
In the present study, we found aberrant expression of the genes relevant to the categories regulation of apoptosis and response to light.
Figure 9b depicts the connections of 13 genes that were contained within one of these GO categories: regulation of synaptic transmission.
To validate the results of Gene Ontology analysis, we evaluated whether the GO categories "regulation of cell proliferation" and "regulation of programmed cell death" were affected by MMG incubation by performing biological assays of T-cell cloning and apoptosis induction.
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This analysis shows that several biological processes were overrepresented among gene targets of these miRNAs: regulation of transcription, development and differentiation, ubiquitin cycle, signal transduction, transport, and tumor suppression (synonym category: regulation of progression through cell cycle) (FDR p<10−9).
Here, we focus on the 42 upregulated genes in the category regulation of apoptosis.
Within the category "regulation of leukocyte activation," ephrin B1 was downregulated, and we therefore examined all ephrins and their receptors.
We found that CHH modifications are highest in the introns of genes in the GO category "regulation of transcription" for both AHB and EHB.
The exception is the GO category, regulation of gene expression, epigenetics, in which the few genes that were detected were only found in exfoliated cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com