It is reasonable to think that the overall carcinogenic effect of tobacco compounds should be measured as the final result of the combined action of the two categories of enzymes.
So far we have shown that different categories of enzymes and metabolic pathways possess well-distinguishable probe-set frequency patterns.
Finally, within the predicted ECs, the prevailing categories of enzymes were transferases and oxidoreductases (31,38% and 29,65% respectively; Additional file 1: Figure S1d).
Accessory proteins which have been hypothesised to possibly have a role in enhancing carbohydrate deconstruction in A. niger were also uncovered in T. reesei and categories of enzymes induced were in general similar to those in A. niger.
The relevant genes encoded four categories of enzymes, including alcohol dehydrogenases (ADH), aldo keto reductases/aldehyde reductases (AKR/ARI), aldehyde dehydrogenases (ALDH), and oxidases as shown in Table 1.
Similarly, the level and pattern of cytosine methylation are established and maintained in plants by interlaced actions of at least three categories of enzymes, i.e., cytosine methyltransferases, active demethylases and several Argonaute proteins (AGOs) related to biogenesis of a specific type of 24 nt small interference (si) RNAs [ 22- 24].
The GO categorization analysis of our anther transcriptome results showed that categories of enzymes, transporters, structural and other molecular proteins were over-represented in the genes with reduced expression, and hydrolases in those with elevated expression levels in ams compared with wild-type.
Thus, we anticipated that the missing piperazate halogenase would be in this category of enzyme.
By employing different filters Sczyrba whittled down the number of the more than two million predicted genes to 27,755 candidate genes that encoded a specific category of enzymes called carbohydrate-active enzymes (CAZymes) that can break down plant polysaccharides (e.g. cellulose) into small sugars.
Phage integrases all fall into a category of enzymes known as site-specific recombinases [13].
