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They have a vast catalog with thousands of identified pieces of debris.
The expressed transcripts were then cross-referenced (Materials and Methods) with the catalog of proteins identified using urea-based or MonoPrep tandem mass spectrometry blastosol analysis.
Figure 8b presents the name of the requirements document on which the concerns were identified and a summary of the identification process, highlighting (i) the list of concerns of the used catalog, (ii) the list of identified concerns, (iii) the amount of software requirements, (iv) the amount of requirements affected by software concerns, among others.
We also assessed them in relation to the mouse imprinting catalog (http://www.mousebook.org/catalog.php catalog=imprinting). Interestingly, 8/32 (25%) of identified miRNA transcripts in the voluntary consumption treatment paradigm, 2/24 (8%) in the T1 paradigm, 13/45 (29%) in the T2 paradigm and 13/68 (19%) in the T3 paradigm mapped to three known imprinted regions of the mouse genome.
For a complete functional cataloging of the identified mutants, we used the GO tool "GO Slim Mapper", further refining the search with annotations of the BIOBASE Knowledge Library Proteome and the Saccharomyces Genome Database (SGD) (Additional file 2).
Currently, there are 2,578 mature human miRNAs listed in the miRBase catalog of human miRNAs (v20, June 2013) [ 22, 29], and the number of identified miRNAs is still rising.
Deep resequencing of this region using next-generation platforms will be necessary to identify the catalog of variation around the identified associations and identify the underlying causative risk alleles, for both the rs7577650/444A signal and the new independent signal we have identified, rs3815676.
The catalog of molecular markers identified here helps unravel this complexity.
These results indicate that GreenGenie2 is an improved ab initio gene-finder for Chlamydomonas and encouraged us to make whole-genome predictions on assembly v3 and compare them to the FGC07 catalog [ 1] with the goal of identifying new genes and improving the accuracy of the current gene models.
We then used the function denovo_map.pl to: (1) assemble the reads of each individual into unique loci (called 'stacks') and identify heterozygous alleles; (2) match orthologous stacks from the surface fish and cavefish P0 into a common catalog of loci and identify SNPs between them; and (3) match the stacks of all F2 against this parental catalog and infer genotypes at all loci.
ApJS 143, 25 45, 2002] catalog have been identified; this is one of the largest sets of optical identifications of such objects thus far.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com