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Important CAT sequence optimization steps include the appropriate choice of the k-space coverage ratio and the application of mixed bandwidth technology.
The first Corman Poes had a certain sly humour that is truer to Poe than his reputation suggests, but he later brought in the knockabout style of his Poe-like A Bucket of Blood for The Raven and the priceless Black Cat sequence in Tales of Terror, in which Price and Lorre are rival wine-lovers mixed up in murder and premature interment.
Despite the overall negativity, Shearman and Pearson noted that the "much derided" cat sequence was not "that badly handled".
Since we had already generated sequences for several jungle cat individuals using the previous primer pair, we truncated the jungle cat sequence length for the cytochrome b region.
For this leopard cat sequence data the General Time Reversible with Invariable sites (GTR+I) model was selected by the AIC using ModelTest.
Of note, the CHR sequence may be aligned with one of the iPLA2 CaM-binding motif (iPLA2618 635), while the CAT sequence aligns with another, i.e. the iPLA2 CaM-binding motif (iPLA2691 709) [41].
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We initially designed a primer set for the cytochrome b region for jungle cats based on house cat sequences.
Primers were designed using existing sequences for the two species as well as from house cat sequences downloaded from NCBI.
Interestingly, a comparison of CHR and CAT sequences with two CaM-binding peptides of iPLA2 reveals marked homologies (Figure 6D).
Initially, we designed primers for the Control Region using domestic cat sequences, since sequences for this region for our study species were not available.
Exclusion of the dog, cattle, and cat sequences from further consideration is also supported by the fact that similar contamination of PCR results with DNA from domestic animals has been reported before and attributed to either handling of the samples or contamination of chemicals [24], [25].
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