The phrase "cat number" is correct and can be used in written English.
It is typically used to refer to a number that identifies a specific cat, such as a registration or identification number. An example sentence using "cat number" is, "Please include your cat's registration number on the adoption form."
The blots were cut in pieces according to the molecular weight of the protein and then incubated with the specific antibodies for MnSOD (1/500, cat number: sc-18503), catalase (1/500, cat number: sc-34285), dynein (1/5000, cat number: sc-13524) and β-actin (1/10000, cat number A5441) in PBS-Tween 20 at 4°C overnight.
An additional control was Allstars negative control siRNA from Qiagen (Cat number: 1027280).
Colonies were stained for alkaline phosphatase (Sigma Aldrich, cat number 86R1KT).
For staining, 20 μL of each of the following human antibodies was added to 100 μL of the cell suspension: CD45-PE monoclonal antibody (mAb); CD73-PE mAb; CD105-FITC mAb; CD90 (Thy1 -FITC mAb; and CD34-APC mAb (Cat: number 304007; Cat: number 344003; Cat: number 323203; Cat: number 328107; Cat: number 343509, Biolegend, California, USA).
Negative control staining was performed using the corresponding isotype control antibodies (FITC, Mouse IgG1, Cat: number 400109; PE, Mouse IgG1, Cat: number 400111; APC, Mouse IgG1, and Cat: number 400119; Biolegend).
Detection was with Vectastatin Elite ABC Kit Vector Laboratoriess, CA, USA Cat number: pk-6101).
Primary antibodies against phosphor-insulin receptor substrate 1 (p-IRS1, Cat. number 2386), IRS1 Catt. number 3407), phosphor-phosphoinositide-dependent protein kinase 1 (p-PDK1, Cat. number 3438), PDK1 Catt. number 5662), phosphor-amino kinase terminal (p-Akt, Cat. number 4060) and Akt Catt. number 4691) were obtained from Cell Signaling Technology.
Antibodies such as anti-iNOS-Alexa Fluor 488 (Cat. number 53-5920), anti-Cat6-PE (Cat. number 12-0862), anti-CD11c-PEc-PE (Cat. number 12-0114) were purchased from eBioscience (California, USA).
Membranes were immunoblotted with the following primary antibodies: p120ctn (BD biosciences Cat. number 610133); SP1 (Abcam Cat. number 58199); E-cadherin (BD biosciences Cat. number 61081); CRK II (Santa Cruz Biotechnology Cat. number sc-289); CRK I/II (Santa Cruz Biotechnology Cat. number sc-17989).
The following monoclonal antibodies were utilized (Becton Dickinson): CD3 (FITC, Cat. number 553062), CD4 (PE, Cat. number 553730), CD8 (APC, Cat. number 553035), IgA (FITC, Cat. number 559354), and CD19 (PE, Cat. number 553786).
Antiglucose transporter 4 (GLUT4, Cat. number G4173) and GAPDH Catt. number G9545) were obtained from Sigma.
