Suggestions(3)
Exact(19)
Then a topological argument asserts that cat ( N ˜ ε ) ≥ 2 cat.
Then the standard Ljusternik-Schnirelmann theory implies that I ε has at least cat ( N ˜ ε ) critical points on N ˜ ε (also see [21]).
Hence the standard Ljusternik-Schnirelmann category theory can be applied in N ˜ ε to yield the existence of at least cat ( N ˜ ε ) critical points of I ε.
About 10 ml mesoglea jellyfish from each population (BsA, JsA, WsA) was cut into pieces and treated with 1 mg/ml collagenase (ICN, cat N 150705) solution in sea water at 37 °C for 30 min.
Geo-referenced (latitude and longitude) unique locations of leopard cat (n = 140) were obtained from museum specimens across the globe, from the current study, from literature [16], [23], [41], as well as locations reported by others authenticated with photographs.
Similar(38)
PCR reactions were performed in triplicates or quadruplets and contained 1× AB gene ABsolute™ QPCR SYBR® Green Mix (Cat. N AB-1166/a AB-1166/aof ROX dye (passive reference dye), 100l of DnM template and 75 nM of each pROXer in a final volume of 25 µl.
Synthesis of cDNA and biotinylated cRNA was performed using the Illumina TotalPrep RNA Amplification Kit (Ambion Cat. n.
Synthesis of cDNA and biotinylated cRNA was performed using the Illumina TotalPrep RNA Amplification Kit (Ambion, Foster City, CA; Cat. n. IL1791), according to the manufacturer's protocol.
Briefly, mouse Atf4 cDNA (OriGene; cat. n. MR29597) was cloned in the exchange vector pPTHC/MCS and then targeted to the Rosa26 locus of the EB3 ESCs, as described.
For mRNA expression profiling, 500 ng total RNA were reverse transcribed, as described previously [ 26, 81] and used for synthesis of cDNA and biotinylated cRNA according to the Illumina TotalPrep RNA Amplification Kit (Ambion, Cat. n. IL1791) protocol.
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