Jayfeather, Hollyleaf, and Lionblaze learn that their true parents are Leafpool and Crowfeather, not Squirrelflight and Brambleclaw; this makes their birth against the warrior code and medicine cat code, codes of honour followed by Clan cats and medicine cats, respectively.
Always remember the warrior cat code!
To investigate promoter proximal and distal transcription, we used anti-RNAPII antibodies and PCR primers specific for the HIVLTR and CAT coding sequences (Figure 4A, top panel).
F-NSpt5, but not F-Spt5, significantly increased the presence of RNAPII on the HIVLTR and CAT coding regions (Figure 4A, compare bars 2 and 5 to bars 3 and 6).
PCR was used to add BamH1, XmaI and XhoI to one side of a 361 bp fragment of the chloramphenicol acetyltransferase (CAT) coding sequence from pSV2CAT (4969–4608 bp Genbank M77788) and XbaI to the other side.
This discriminatory effect was also observed with another flavagline, compound #7 (Fig. 1A), as well as on another set of reporter constructs in which the highly structured HIV TAR element was placed upstream of the chloramphenicol acetyl transferase (CAT) coding region (Fig. S5A).
To explore whether an EJC deposited on an AUG codon may negatively affect initiation at this site, we prepared three sister constructs that contain an intron at different locations, such that an EJC is deposited on AUG1, AUG2, or in the CAT coding region.
pΔ(−71 SomCAT contains the somatostatin promoter to position −71, fused to the chloramphenicol acetyl transferase (CAT) coding sequences (Montminy et al, 1986).
SCA1 disease alleles of the ATXN1 gene all contain uninterrupted tracts of CAG repeats while virtually all normal alleles have one to three CAT (coding for histidine) interruptions in the middle of the Q-tract [ 39].
For example, normal ATXN1 has one to three CAT (coding for histidine, H) interruptions near the middle of the Q-tract, but in SCA1 disease alleles the repeat tracts are pure CAGpolyQ [ 39].
To analyze the promoter regions of the sigH- rshA operon and of the rshA gene, DNA fragments (504 bp upstream of sigH and 301 bp upstream of rshA) were cloned in the promoter probe vector pET2, thus forming transcriptional fusions of the promoter-active fragments and the reporter gene cat coding for chloramphenicol acetyltransferase (CAT).
