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Gel casting, tissue homogenization and solubilization, electrophoresis and immunostaining were as previously described.
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Or they simply cast "tissue ballots" – dozens of thin tickets folded together to look like one.
A total of 0.1 ml chitosan solution/cm was cast into tissue culture-treated plates and dried over-night in an incubator at 37°C.
In contrast, recent evidence has reported a lack of expression in normal breast tissues [ 18], casting doubt on the breast tissue as the main source of PSA in human females.
Soft tissue, cast model and perioral muscular electromyography data analyses were also to be performed, if data were available.
Other interventions involve cast disruption by tissue plasminogen activator or urokinase and prevention of cast formation by use of mucolytic agents, steroids, or anticoagulants.
For tissue culture, collagen monolayers were cast in 6-well tissue culture trays with each well containing 800 μl collagen and 200 μl of the neutralization solution (2 parts 0.34 N NaOH and 3 parts 10× Waymouth media) to a final concentration of 1.9 mg/ml.
Ms. Grenke, a senior at the time, was casting for a school production of "Tissue," a play by Louise Page, a British playwright, about a woman with breast cancer.
Thank God he does, because it's one of the only moments in the film I can cast aside my tissues and cynically raise my eyebrows.
Recently, it has become widely accepted that PSA is also present in many nonprostatic sources, casting doubts about the specificity of its tissue expression.
One avenue has involved casting molds of blood vessels, and then culturing tissue around them.
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