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The "currently" is key because even though the studio's casting grid lists Gordon-Levitt as The Riddler, the movie is in such pre-production that Christopher Nolan could freak out and demand, say, Shia LaBeouf play The Riddler.
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The total surface area counted was determined using the Cast Grid software (Olympus, France).
The analyses were done with Cast grid software (Olympus).
We counted the immunopositive cells using the Computer-Assisted Stereology Toolbox (CAST) Grid System (Olympus, Copenhagen, Denmark) on a light microscope (Olympus BX S1).
The total weight of grids and top lead used in a battery can be reduced by 40% compared with conventional cast grids.
Cells were counted using an AX70 Olympus microscope equipped with a digital color camera, an x y motorized stage controller, a microcator to measure stage movements along the z-axis with a precision of 0.5 µm, and CAST-GRID stereology software (Olympus, Denmark).
The resorbed area was measured using CAST-Grid (Olympus).
The software used was CAST-GRID v 1.09, developed by Olympus, Denmark.
BrdU-positive cells were counted (double-blinded) and scored using Olympus CAST-Grid stereology and Fractionator method.
A camera transmits the image to a computer screen where a counting frame is superimposed using the computer-assisted stereological (CAST -Grid software (Visiopharm, Hørsholm, DK).
Using a light microscope with motorized stage and the CAST-grid analysis software, the volume fractions of tubules and the volume fraction of the interstitial space in the cortex were estimated by point counting in eighty systematically randomly sampled areas from each individual animal [ 14, 15].
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