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Their spinal cords were then immersion-fixed in 4% paraformaldehyde (4% PFA/0.01M PBS) for 4 h, cryoprotected in 30% sucrose and cast frozen in O.C.T. (Tissue Tek, EMS, Hartfield, PA) for cutting on a Cryostat.
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The cast froze.
2. The cast froze their butts off during those nighttime beach shoots, because Southern California beaches are NOT warm at night.
When it casts "Freeze" at you, eat a lobster to un-freeze so you have time to run off or fight.
Minutes later, the cast freezes, and a narrator puts the decision to a vote by audience applause.
A 'living hell' of a shoot, according to whistleblowers, with reports of the cast freezing in the Canadian wilderness and multiple crew members quitting under the tough conditions.
There are times when the cast seem frozen in time, like human flies preserved in amber.
The implicit optimism of such scenes is given the lie by other group tableaux in which the cast stands frozen in grim postures of sorely tested patience.
Ponder the inevitable mid-summer fog that has cast a freezing blanket over the city.
A dispersion of collagen is then cast into the mould and frozen.
Perhaps she's been somehow stymied by those inert golden sculptures hanging from the rafters: the cast is often frozen in place as well, arrayed in static stage pictures that convey little of the flavor or complexity of the relationships in the play.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com