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Fatigue tests using the CoAxial Shear Test (CAST) apparatus were performed in both dry and wet conditions with and without temperature cycles.
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A multi-gel casting apparatus for simultaneous casting of homogeneous concentration and gradient concentration sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) gel was described here.
To select this composition (i.e., the Ti66Fe20Nb8Sn6 alloy), three Ti-Fe-Nb based chemistries (Ti63Fe23Nb8Sn6, Ti60Fe23Nb8Sn9 and Ti66Fe20Nb8Sn6) were originally generated under bulk conditions by using a suction casting apparatus.
Down on the floor, another circle mirrored the one above, with incandescent bulbs illuminating transient puffs of smoke and casting the apparatus in a ghostly light.
The 2D was resolved on a 1× Tris/glycine gel (Biorad) and proteins separated by size (molecular weight) in a direction perpendicular to the first dimension run on the Protean 2D casting and running apparatus.
SDS-PAGE (BIO-RAD TGX acrylamide) gels were loaded with 8 μL of protein extracts in loading buffer and typically run for 0.5 hr at 206 V using the BIO-RAD casting system and electrophoresis apparatus (Mini-PROTEAN Tetra Cell).
After PCR amplification, 8 µl of the amplification products were electrophoresized in a non-denaturing 6% polyacrylamide gel casted in a sequencing apparatus (cm 21 x cm 40 x mm 0.4; Sequi-Gen, Bio-Rad, Richmond/USA) at constant 55 W and the band pattern visualized by silver staining as reported in Promega's manual Silver Systemce-DNA Sequencing System (13).
I hope the LDP apparatus can be cast off before that.
The PCR product was resolved onto a 6% denaturing gel containing 7 M Urea cast on the BioRad midigel apparatus.
The Guardian splashes on the news, saying Obama cast his scrapping of the legal apparatus set up by George Bush as a way for America to reclaim the moral high ground in the fight against al-Qaida.
A new-developed apparatus made possible to cast the repair concrete under water on the previously prepared concrete substrate and use hydrostatic pressure 0.5 MPa.
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