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There are 174 gene-level abundance estimates that differ by >50% from ground truth for the CAST alignment, vs. 485 such estimates after alignment to NCBIM37.
For most of this cohort (2242 of 2984 genes) the simulation results show that the CAST alignment abundance estimates are closer to the ground truth (green circles in Figure 3A).
If we consider only perfect-matching reads (reads that align with zero mismatches), only 24% of gene estimates from the NCBIM37 alignment are within 10% of ground truth compared to 79% of gene estimates from the CAST alignment.
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The difference between alignments is more striking for perfectly mapping reads (zero mismatch), as 23% more CAST reads (+982,229) align perfectly to CAST but not to NCBIM37.
The novel cast maintains fracture bone alignment and immobilises the forearm during the healing process.
The search for a common local pattern among a set of RNA sequences can be cast as a multiple local alignment problem, where the alignment takes into account both sequence similarity and base-pair correlation.
Alignment of simulated CAST reads to the CAST transcriptome improves the accuracy of gene-level abundance estimation compared to alignment to NCBIM37 (Table 3).
To support very low overlap cases, the CPSH-based alignment is furthermore cast into a probabilistic framework that involves computing the CPSHs on segments of the point clouds and finally fused using an ML estimator.
A total of 458,297 reads improve by alignment to the CAST transcriptome (Table 2, italic entries), of which 93% (427,016 of 458,297 reads) improve by two or more categories (Table 2, underlined italic entries).
Compared to CAST, more B6 reads align with fewer mismatches to fewer positions in the transcriptome, resulting in increased alignment specificity.
How the Arab Spring evolves, and how far the protests spread in the Arab world and beyond — as old assumptions about geopolitical alignments are being cast aside — are among the big uncertainties of 2012.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com