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At college, Matt's first band played Police covers and sold cassettes outside the gates, but the proper action started with his first two serious bands, the Supergrass-like Ruth and the 45s.
"Everybody's always grateful when I give them free tapes," said Julian Trevino, a college freshman, who was distributing Ultraspank cassettes outside the San Antoni concert by Slipknot, a band that Mr. Trevino, by the way, discovered when someone gave him a Streetwise cassette sampler of the band.
Regardless of mechanism, dual vectors utilizing either strategy are becoming a viable option for expression cassettes outside the traditional packaging limit of AAV.
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For transfections, the plasmids were digested with XbaI, in order to linearise them outside the neomycin resistance cassette and outside the PTEN coding sequence.
Chinese makers are expected to continue selling Sony-branded cassette Walkmans outside Japan, i.e. in Asia and the Middle East.
There is one clear-cut example of cassette insertion outside an integron emerging from the analysis of the DAT722 array.
In combination with a specific primer which binds inside of the selectable marker (pyr4) and the primer which binds outside from the cassette we were able to very efficiently screen the mutant strains by PCR.
In addition, many of them promote their music the way hip-hop acts do, using street teams of fans who work free, dispensing cassettes and stickers at schools and outside concerts.
Transcriptional silencing results from the presence of the bacterial plasmid backbone or virtually any DNA sequence of >1 kb in length placed outside of the expression cassette.
For each gene we designed a specific primer which binds outside from the deletion cassette in the flanking regions of the gene (Additional file 1 Figure 1B).
CDC also performed confirmatory testing on fungal specimens from other laboratories, including a fungal isolate recovered by FDA from a BBG vial and fungal DNA isolated by an outside laboratory from a vitreous cassette specimen.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com