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Finally, we characterized the gene cassettes associated with integrons in Methylobacillus flagellatus KT and Dechloromonas aromatica RCB, and found a heavy metal efflux gene as well as genes involved in protein folding and stability.
The number of active toxins is positively correlated with the total number of toxin cassettes, suggesting that with an increase in the number of individual polymorphic toxin loci the number of toxin cassettes associated with them increase more or less linearly (Additional File 1).
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Given that cassette associated genes are part of the gene pool that is LGT-associated and mobilizable, they also represent a community resource that can be shared between individuals [ 11].
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Those mic elements are not related to the gene cassette associated with class 1 integrons (26 ).
It seems that the authors have observed a circular form containing the gcu14 gene cassette associated with the aacA4 gene cassette.
The first of these is the cassette associated 59-be [ 4] and the second is a site contained within the integron designated attI.
Variants of the aacA4 [also called aac(6' -Ib ] cassette associated with resistance to either gentamicin (C at position 329, encoding serine) or amikacin (329T, encoding leucine) (17 ) are called aacA4 -C329 and aacA4 -T329.
The latter scenario of cassette insertion outside an integron context has been previously documented for gene cassettes generally associated with class 1 integrons [ 3].
The tpsC cassettes for recombination can be acquired from the environment and recombine into the tpsA gene or in the downstream region, potentially resulting in an increased or decreased number of tpsC cassettes and associated IORFs.
MHCI pathway molecules Sec61a1 (an endoplasmatic reticulum membrane protein translocator involved in crosspresentation to CD8 T cells) and TAP-1 (ATP-binding cassette transporter associated with MHCI loading) were expressed on cDCs and on both IKDC and B220−NK cells.
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