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In the two reagent addition positions, the fluid delivery needles output directly to the underside of the cassette where the reaction vessel is sealed for reagent addition.
To direct FVIII expression to the megakaryocyte lineage, we designed a FVIII expression cassette where the human B-domain deleted FVIII cDNA was placed under the control of the megakaryocytic/platelet-specific glycoprotein IIb (αIIb) promoter.
In mouse, a Sult1c–(Mark)–(Ck)–(Mark)–(Hcfc1r1)–(Sult1c) gene cassette (where names in brackets are pseudogenes) has been duplicated and a subsequent inversion event between Sult1c and the first Mark pseudogene rearranged one copy of the cassette.
Consider using controls for genetic manipulations of lncRNA loci: inverting the truncation cassette where possible, using a mutated truncation cassette, using a different type of truncation cassette, and using different sites to truncate the lncRNA.
In pFKPEN, recombinant FkpA (rFkpA) expression is controlled by its native promoter, while pFKPEI contains a dicistronic expression cassette where rFkpA gene transcription is controlled by the lac promoter.
It comprises of three independent expression cassettes, a) one for the expression of CymR, using the CMV5 promoter, b) a second cassette where the CMV promoter drives BFP expression.
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I leave all the Natalie Merchant cassettes where they are.
Of the 1 787 cassettes where the collapse could be used for verification, the borders were supported in 1 318 (74%) cases.
The KASPar system uses two Förster resonance energy transfer (FRET) cassettes, where fluorometric dye is conjugated to the primer but quenched via resonance energy transfer.
These promoter:: cSUC2 cassettes where then subcloned into pGPTV-Bar [ 30], as is or as fusions with GFP and uidA, and electroporated into Agrobacterium tumefaciens strain GV3101mp90 as described [ 31].
A previously unknown cassette structure, where the TR and RT are located on different strands (see below), was classified as group 4. Within those groups, we observed a large variety of arrangements.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com