Purified proteins were dialysed overnight at 4°C in 25 mM ammonium acetate using a Slide-A-Lyzer Dialysis Cassette, 10 K molecular weight cut-off (Thermo Scientific) and stored at −20°C.
The excess formaldehyde was removed by dialysis against one change of PBS, pH 7.4, and three changes of Tris acetate/EDTA (TAE) buffer, pH 4 (40 mM Tris, 1 mM EDTA) in Slide-A-Lyzer dialysis cassettes with a molecular weight cut-off of 3.5 kDa (Pierce, Rockford, IL, USA), as previously described [25], [26].
Slide-A-Lyzer dialysis cassettes with a molecular weight cutoff of 10000 were purchased from Thermo Scientific (Rockford, IL).
Purpose: To model the EC-L portal film cassette to understand how its weight could be reduced without compromising image quality.
LNPs were placed into a 10 000 molecular weight cellulose dialysis cassette and dialyzed overnight at 4 °C against phosphate buffered saline (PBS) pH 7.4.
Peak fractions were pooled and dialyzed against PBS in 10,000 molecular weight cutoff dialysis cassettes (MWCO Thermo Scientificic) to remove excess HA peptide.
Virus was then concentrated 100 fold, using a Labscale tangential filter flow apparatus connected in parallel with two Pellicon XL 50 Cassettes with 1000 kDa molecular weight cut-off (Millipore, Billerica, MA).
A weight-bearing long cassette (including hip, knee, and ankle bilaterally) lower extremity joint survey was obtained at six weeks, and anteroposterior, lateral, and sunrise radiographs were obtained at six weeks, six months, one year and biannually thereafter.
To remove DMSO and residual vancomycin, VPA(X) derivatives were purified by 24 h dialysis against 1 M NaCl followed by 24 h against deionized H2O using 3,500 molecular weight cutoff dialysis cassettes.
With the help of cassette blanks the imprecision in dust weight could be held below 0.05 mg, if the cassettes were allowed one week's storage in the weighing room before weighing, both before and after sampling.
