Sentence examples for cassette up from inspiring English sources

In each transformation event, in addition to the selection marker and/or the crtS gene expression cassette, "up" and "down" DNA fragments were included targeting the insertion into the integration site.

The encoded ATPase domain-containing proteins exhibited significant sequence diversities, not only between the three types of cassettes but also within the same cassette (up to 49% amino-acid sequence identity, but in most cases less than 40%), indicating a complex evolutionary history of the gene cassettes.

A 4 kb Plst-4 promoter region and 847 bp lst-4 3'UTR and downstream region were amplified from genomic N2 wild type DNA using primers which added for Plst-4 a SbfI and an AscI cassette up- and downstream and SpeI and ApaI cassettes for lst-4 3'UTR respectively.

He believed in this working-class idea of self-improvement and discovered a love of opera – to the extent that he took cassettes up on the roofs of building sites to play them, which was incredibly embarrassing for me as a 16-year old punk.

In order to maximize the efficiency and versatility of the vector-based siRNA approach, using an efficient cloning strategy we have developed a series of siRNA expression vectors containing multiple tandem siRNA cassettes (up to six).

Through the sequential introduction of these vectors into the cell, we achieved highly accurate genome modifications, including nucleotide substitution, gene deletion and cassette insertion up to 3.6 kb.

Archaeal and bacterial genomes contain cassettes of up to multiple CRIPSR units, in some cases, more than one cassette per genome.

This scaffold was used to create cassettes containing up to four Broccoli units, markedly enhancing the brightness of mammalian cells expressing cassette-tagged RNAs.

In the United States, sales of albums, as the industry anachronistically calls full-length CD's and cassettes, are up about 8percentto to 312 million through mid-June, according to Soundscan, which tracks such things.

A recent description of the canine T cell receptor γ locus identified a large number of formerly unknown genes, and determined the locus topology consisting of 8 cassettes with up to 3 variable (V) genes, 2 joining (J) genes and one constant (C) gene each.

These combinations yield relatively long expression cassettes (up to 3.7 kb in the case of FLuc-RFP-TK sequence) and suffer loss of activity for the reporter downstream of the IRES element [[18]].