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A separate viral fraction was concentrated by tangential flow filtration (TFF) using a Pellicon housing (Millipore, Bedford, MA) fitted with a Biomax-50 (polyethersulfone) cassette filter (50 Kda pore size).
One liter of each concentrated sample was obtained after retention through a Durapore 0.1 μm PVDF filter (Pellicon 2 Cassette Filter, Screen type C, size 0.5 Milliporepore Corporation, MA, USA).
At each sampling site, 180 l of samples were collected and pre-filtered through a 5.0 μm SMWP membrane (diameter 290 mm; Millipore, Ireland) to remove suspended particles, then filter-concentrated with Tangential Flow Filtration (TFF) system through Durapore 0.1 μm PVDF filter (Pellicon 2 Cassette Filter, Screen type C, size 0.5 m, Millipore Corporation, MA, USA).
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4-mer distribution analysis of barcode-cassette filtered metagenomic sequences of serially diluted gut microbiota.
The passive monitor, which has been previously described, samples nicotine from ambient air.[ 26, 27] A 4-cm-diameter polystyrene cassette holds a filter treated with sodium bisulfate and a membrane filter functions as a windscreen.
An A-P X-ray is taken from the hips to the ankles with the patient standing and the patellae looking forward using a suitable cassette to gradually filter the X-ray beam in order to visualize both hips and ankles properly.
A high-volume air sampler was used in parallel with a filter cassette to evaluate spiked samples and unspiked samples.
In summary, the samplers consist of a 37-mm-diameter plastic cassette containing a filter treated with sodium bisulfate.
The samplers consisted of a 37-mm-diameter plastic cassette containing a filter treated with sodium bisulfate.
Then, air samples were monitored during ADS seasons using a filter cassette coupled with a real-time quantitative polymerase chain reaction (qPCR) assay.
The air samples were collected using a filter cassette connected to a personal sampler (pump) running at 4.0 liters per minute.
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