The construct was then recombined (according to the manufacturer's instructions) into the Agrobacterium binary vector pWBVec8 [ 46] modified to contain a Gateway cassette down-stream of the Ubiquitin promoter.
Early improvements included the addition of a second barcode, designating them "up" tags (5′ to the KanMX cassette) and "down" tags (3′ to the KanMX cassette), providing a hedge against barcode failure (usually the result of error introduced during chemical synthesis of the oligonucleotides) (Eason et al. 2004; Smith et al. 2009).
In each transformation event, in addition to the selection marker and/or the crtS gene expression cassette, "up" and "down" DNA fragments were included targeting the insertion into the integration site.
It was 13 years ago, also during the holiday season, that a small amount of such material, believed to have been hidden inside a radio-cassette player, brought down a Pan Am 747 over Lockerbie, Scotland.
She set down a cassette tape recorder in front of her saba, her grandfather, and pressed a button.
It has been just over a month since Muxtape, a popular music site that let users share the online equivalent of cassette mixtapes, was shut down by the RIAA for copyright infringement issues.
A couple of these songs were laid down on cassette just once and we had to really honor that iteration and try to coax more from it.
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Armed with a synthesiser, a drum machine and a cassette deck, Larry Heard laid down "Can You Feel It," a Ron Hardy approved house record that still sounds as fresh today as it must have done during the Reagan years.
DLP and AP were cut longitudinally through the urethral part of the prostate and both tissue pieces were put in the cassette with the cutting area facing down.
I stopped him in his tracks and got down a cassette from the performance of Memorial and said: 'Do you mean something like this?' He was startled and said: 'That's exactly what I want.' He said: 'Can you make me another piece like this?' and I said: 'No.
