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Briefly, a cassette containing the human DTR open reading frame was placed upstream of the initiation codon of Agrp in the first exon.
The Guinea Pig EP is the band's debut album, though they've also released an eponymous cassette containing nine tracks.
These clones harbored a DNA cassette containing two heterologous ORFs (cDNAs) or three heterologous ORFs in tandem downstream of a single promoter.
To knockdown CstF-64 and CPSF-73, a cassette containing the mouse U6 promoter and the specific shRNA sequence was excised from pBluescript and subcloned into pLenti6 (Life Technologies), as described previously51.
The expression cassette containing MCR1 was integrated into GSE16 after PCR amplification using primers YLR446W-F and YLR446W-R.
Plasmid pET28a-IPaA had an expression cassette containing IPa and celCCA at the N and C termini, respectively.
Plasmid pET21a-ScafBAC had an expression cassette containing IPA flanked by IPB and IPC at the N and C termini, respectively.
Specifically, cDNAs of α-, β′-, and ε-COP were inserted into one expression cassette containing a p6.9 promoter and a SV40 terminator, while cDNAs for the other four subunits (β-, γ-, δ-, and ζ-COP) were inserted into another expression cassette containing a p10 promoter and a SV40 terminator.
But they did not take a cassette containing tape recordings of the deputies' calls from the house following the shooting.
STOP is a transcriptional and translational stop cassette containing multiple poly-adenylation signals.
Finally, the two PCR products were used in a third PCR to create a cassette containing maC46 fused to GFP.
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