Sentence examples for cassette coding from inspiring English sources
The resulting plasmid with both Xac1814 flanking regions was digested with HindIII and in this site the 2 kbp Ω cassette coding for Sm/Sp resistance from pKRP13 [44] was subcloned.
The resulting plasmid with both Xac1815 flanking regions was digested with BamHI and in this site the 2.3 kbp aacC1 cassette coding for Gm resistance was subcloned rendering pK195 aacC1 3Xac1815. E. coli S17-1 cells transformed with pK195Ω3Xac1814 and pK195 aacC1 3Xac1815 were conjugated to X. axonopodis pv.
The tCD2-luc2 protein also opens interesting perspectives for use in multi-cassette vectors, for example vectors containing one cassette coding for a shRNA combined to a cassette coding for a tracer protein.
Another gene in these strains, URA3, had been substituted with the kanMX4 cassette coding for selectable marker resistance to kanamycin (Cubillos et al. 2009).
This band was undetectable in DNA from parasites transfected with both the knockout and complementation plasmid (lane 4), indicating that the gene can be disrupted only when an additional cassette coding for the PfCK2β1 subunit is provided to the parasites.
Subsequent replacement of HO by the hphMX4 cassette (coding for resistance to hygromycin B), followed by sporulation and tetrad dissection, produced isogenic haploids of stable a or α mating type.
VIM-2 confers resistance to the majority of β-lactams and is associated with other gene cassettes coding for enzymes that inactivate aminoglycosides.
Integrase sequence can insert many cassettes coding for different antibiotic resistance, therefore, making a single bacterium possessing it to be resistant to multiple antibiotics.
The corticosteroid receptor LBDs were each subcloned in frame with the GAL4 DBD in pBluescript KS- to produce gene cassettes coding for single FLAG-tagged GAL4 - LBD fusion proteins.
Most (46%) of the VIM producers belonged to the internationally-distributed clusters of ST111 and ST235, which carried previously-reported gene cassettes coding for the VIM-2 and VIM-4 enzymes, respectively.
Three genes in the bacteriocin cassettes coded for putative CAAX amino terminal proteases: blpY and pncP, located at the end of every cassette, and a novel gene (blpG) within the BIR of prototypes 30 – 30.
