Sentence examples for cassette amplification from inspiring English sources

After cassette amplification, all amplified DNA fragments were purified, cloned, and sequenced.

Primers sequences for cassette amplification and verification were obtained from Euroscarf.

The eGFP-pSV40-NeoR reporter cassette, amplification protocols and primers were kindly provided by Dr Yvonne Fischer Fischerr et al., 2010).

Primers for cassette amplification and verification were obtained form Euroscarf. 2) preparation of a replacement cassette containing PDR8-loxP-kanMX4-loxP by the insertion of loxP-KanMX4-loxP into the terminator of PDR8 in strain S288c (primers are given in Additional file 4: Table S4).

Primers sequences for cassette amplification and verification were obtained form Euroscarf. 2) allelic switch by depletion of the kanMX cassette by WAR1 sequence form S288c DNA (primers in Additional file 8) and selection on YEPD containing 300mg/l sorbic acid, pH4.2.

When TS-jPCR was conducted with the tailed mock DNA fragment and the Ig-cassette, no amplification product was detected.

Recombinant MHV-68 BACs were characterized by restriction digestion combined with Southern analysis, as well as direct DNA sequencing of the Cre expression cassette following PCR amplification from the reconstituted viral genome.

Instead, ends can be randomly chosen because the unique blunt end cleavage of the Mly I restriction enzyme recognizes and removes the suicide cassette added for amplification purposes.

The following Supporting Information can be found in the online version of the article: Figure S1 Construction of the chromosome VI with new amplification cassette.

Yeast strain LS20 was used for a host strain, into whose chromosome the amplification cassette was integrated (Butler et al. 1996).

Oligonucleotides cre5'F and Cre5'Rtailhph were used for the promoter region, cre3'R and Cre3'Ftailhph for the terminator region, and M1LHhph and M2LHhph for the hygromycin B expression cassette [ 45] amplification.