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Cluster stability testing detected no more than 5/75 unclassifiable cases to be excluded (7%).
Checking for completeness of both the Postresuscitation care and Preclinical care datasets required 127 Postresuscitation care cases to be excluded from further analysis, leaving 584 cases with complete data for final analysis.
Missing data patterns were analysed by preparing a missing data report (% missing) for first-order questions (no skip patterns or secondary data) to identify data elements that required further work and cases to be excluded if more than 50% of data was not retrieved across all settings.
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A total of 381 cases, 377 HC children and 380 CC children were recruited; 4 cases had to be excluded from the statistical analysis because outcome information was missing, reducing this cohort to 377 children.
Seven cases had to be excluded from the analysis due to inconsistent dates of ART initiation and follow-up outcome.
Owing to missing baseline marker levels, five cases had to be excluded from statistical analysis.
After pathological review, 37 cases had to be excluded because of insufficient tumour cells or only containing carcinoma in situ.
Only 7 out of 384 cases had to be excluded for the reasons given above (1.82%), resulting in 46 to 48 data points per factor combination.
Hence, a very large number of cases had to be excluded from the study, because they failed to meet this important criterion.
However, seven subjects among these 93 cases had to be excluded from the analysis due to missing data at T2 on study variables of interest (sample loss: 30.6%).
Unfortunately, almost 100 cases had to be excluded from analysis as they did not meet the necessary requirement for a heterozygous SNP locus in the PLAC4 mRNA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com