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Among 50 LADCA cases, the response of gefitinib treatment was evaluated in 22 cases (responder (PR), n=15; nonresponder (SD), n=7; Supplementary Table S3).
HSP60 and HSP27 staining are shown for two representative cases (responder and non-responder) in Figure 2. Quantitative real-time PCR analysis was performed on 88 biopsies; in two cases, mRNA extraction was not successful.
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In Bcl-2-negative cases, responders to immunotherapy showed a better prognosis than non-responders (P=0.0575).
Some responders selected more than one reponse for some questions; in most of these cases, responders chose two options that were similar to one another.
However, clinical trials with these agents revealed that in most cases, responders harbored specific activating mutations in exons 18 21 which collectively encode the kinase domain of the EGFR gene [ 2- 5].
Taking into account the higher response-rate among cases, the responder analysis does not point to substantial differential selection bias.
In contrast, anti-leukaemic cytotoxicity was shown in 12/13 cases when responder lymphocytes were stimulated with both autochthonous leukaemic and unrelated allogeneic normal lymphocytes.
In cases where responder and remission data was not reported, it was imputed from means and standard deviation using the method described by Furukawa et al. [ 23].
Significantly more cases of responders had low expression of XIAP compared with non-responders (90% vs 35%, P<0.01).
In some cases, first responders were hesitant to mix with other agencies if they had not trained together, he said.
In a few cases, first responders heard faint cries from under rubble, but shortly those whimperings stopped.
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