This may explain as to why only 61.2% (199 of 325) of our samples were evaluable by MS-PCR and why only in 75% of the cases replicate experiments on 20 randomly selected tumor samples yielded reproducible results.
Identical conditions were used for all proteins tested, and in most cases replicate data sets acquired (Table S1).
The 50/50 case replicates the equivalent condition of the previous experiment: Specific errors constituted 9% of errors, categorical errors 23% (t(11) = 7.5, p < 0.0001).
In each case, replicate microarray hybridizations were carried out such that a p value could be calculated for each difference in gene expression.
Association of rs1344706 in the ZNF804A gene (2q32.1) with schizophrenia was first reported in a genome wide scan conducted in a sample of 479 cases and replicated in 6666 cases.
In some cases, replicate experiments were completed.
In all cases, replicate sample well CT values never deviated more than 0.5% from one another, lending high credence to the technique's consistency, stability and reproducibility (Figs. 9 and 10).
However, the overall pattern of hyperactivity/impulsivity scales being relatively better at differentiating ADHD from non-ADHD cases was replicated in the discrimination between ADHD and SLI.
If our finding that rare missense GNB1L variants contribute to ASD in a subset of cases is replicated, studies would be warranted to determine the effects of such mutations on the GNB1L protein whose function is not well understood.
