In both cases, rats voluntarily and repeatedly dosed themselves with the hormone.
In most cases, rats stare at novel pictures, then gradually lose interest as the images become familiar.
Nonetheless, in both cases rats are suboptimal, i.e. the behaviour they adopt does not yield the best possible outcome.
However, in two cases (Rats 2 and 5), the shifts along the pulse-frequency axis were substantial (−0.18 common logarithmic units), albeit much smaller than the corresponding shifts along the price axis (0.41 and 0.30 common logarithmic units, respectively).
In some cases, rats were anesthetized with propofol [15 mg/kg body weight, i.p. (intraperitoneal) injection], instead of sevoflurane, for comparison.
In all cases, rats were euthanized by excess isoflurane, and their brains rapidly harvested, frozen in methylbutane at −30 °C and stored at −80 °C for subsequent immunohistochemistry performed on cryostat-generated 12- μm sagittal brain sections mounted onto glass slides.
Behavioral experiments confirm that, at least in some cases, rats are able to return to their starting position, even if they were exploring the environment in complete darkness, devoid of smell (in a water platform or a water maze), and without whiskers to orient towards walls, simply by using their motor signals and vestibular system (Cheung et al. 2012).
Thus, in both cases, rats with low scores on factor 1 reared a lot in the cLDB, OF and EPM, made many line crossings in the OF, and swam a relatively short distance before finding the submerged platform in the sMWM.
From a family of chillies that translates as "mouse-shit chillies" or, specifically in this case, "rat's turd", they have an, err, knobbled appearance – small and relatively innocent-looking.
To assess whether this was the case rat cortical neurons were cultured in Neurobasal medium for seven days.
